FUNCTION: Catalyzes the formation of the isocyclic ring in chlorophyll biosynthesis. Mediates the cyclase reaction, which results in the formation of divinylprotochlorophyllide (Pchlide) characteristic of all chlorophylls from magnesium-protoporphyrin IX 13-monomethyl ester (MgPMME) (By similarity).
CATALYTIC ACTIVITY: Magnesium-protoporphyrin IX 13-monomethyl ester + NADPH + O₂ = 13¹-hydroxy-magnesium-protoporphyrin IX 13-monomethyl ester + NADP⁺ + H₂O.
CATALYTIC ACTIVITY: 13¹-hydroxy-magnesium-protoporphyrin IX 13-monomethyl ester + NADPH + O₂ = 13¹-oxo-magnesium-protoporphyrin IX 13-monomethyl ester + NADP⁺ + 2 H₂O.
CATALYTIC ACTIVITY: 13¹-oxo-magnesium-protoporphyrin IX 13-monomethyl ester + NADPH + O₂ = divinylprotochlorophyllide + NADP⁺ + 2 H₂O.
COFACTOR: Iron (By similarity).
PATHWAY: Porphyrin metabolism; chlorophyll biosynthesis (light-independent).
SIMILARITY: Belongs to the acsF family.

Copyright

Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.

Cross-references

Sequence databases

EMBL

CT971583; CAK23528.1; -; Genomic_DNA.

[EMBL / GenBank / DDBJ] [CoDingSequence]

RefSeq

YP_001224825.1; -.

3D structure databases

ModBase

A5GKR3.

Ontologies

GO:0005506;	Molecular function: iron ion binding (inferred from electronic annotation from HAMAP).
GO:0048529;	Molecular function: magnesium-protoporphyrin IX monomethyl ester (oxidative) cyclase activity (inferred from electronic annotation from HAMAP).
GO:0015979;	Biological process: photosynthesis (inferred from electronic annotation from HAMAP).
QuickGo view.

Family and domain databases

HAMAP

MF_01840; -; 1.
PBIL [Tree]

InterPro

IPR008434; AcsF.
IPR003251; Rubrerythrin.
Graphical view of domain structure.

Pfam

PF02915; Rubrerythrin; 1.
Pfam graphical view of domain structure.

TIGRFAMs

TIGR02029; AcsF; 1.

BLOCKS

A5GKR3.

Genome annotation databases

GeneID

5147809; -.

GenomeReviews

CT971583_GR; SynWH7803_1102.

KEGG

syx:SynWH7803_1102; -.

CMR

A5GKR3; SynWH7803_1102.

Other

ProtoNet

A5GKR3.

UniRef

View cluster of proteins with at least 50% / 90% / 100% identity.

Keywords

Chlorophyll biosynthesis; Complete proteome; Iron; Metal-binding; NADP; Oxidoreductase; Photosynthesis.

Features

Feature table viewer

`Key`	`From To`	`Length`		`Description`	`FTId`
`CHAIN`	`1 360`	`360`		`Magnesium-protoporphyrin IX monomethyl ester [oxidative] cyclase.`	`PRO_1000070553`

Sequence information

Length: 360 AA [This is the length of the unprocessed precursor]

Molecular weight: 41422 Da [This is the MW of the unprocessed precursor]

CRC64: 66465D9F7D744B03 [This is a checksum on the sequence]

        10         20         30         40         50         60 
MVPPTAVNDA PAAGSAVSVK DPAKDTILTP RFYTTDFEAM AAMDLRPNEA ELEAICEEFR 

        70         80         90        100        110        120 
KDYNRHHFVR NDEFEGAADK LDPETRRVFV EFLEQSCTSE FSGFLLYKEL SRRIKTRNPL 

       130        140        150        160        170        180 
LAECFAHMAR DEARHAGFLN KSMSDFGLQL DLGFLTANKS YTFFQPKFIF YATYLSEKIG 

       190        200        210        220        230        240 
YWRYITIFRH LEKNPDSKIF PIFNFFENWC QDENRHGDFF DALMKAQPNT VRGFRARLWC 

       250        260        270        280        290        300 
RFFLLAVFAT MYVRDVARKE FYEALGLDAR DYDRLVIDKT NETTARVFPV VLDVKNPKFY 

       310        320        330        340        350        360 
AGLENLVTNN AALDAVDASA SSAPIKVLRK LPHWIANGAQ MASLFLMAPV RSESFQPSVR

A5GKR3 in FASTA format

View entry in original UniProtKB/Swiss-Prot format
View entry in raw text format (no links)
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	BLAST submission on ExPASy/SIB or at NCBI (USA)		Sequence analysis tools: ProtParam, ProtScale, Compute pI/Mw, PeptideMass, PeptideCutter, Dotlet (Java)
	ScanProsite, MotifScan		Submit a homology modeling request to SWISS-MODEL
	NPSA Sequence analysis tools