ID   ACSF_BRASO              Reviewed;         353 AA.
AC   A4YNQ1;
DT   05-FEB-2008, integrated into UniProtKB/Swiss-Prot.
DT   29-MAY-2007, sequence version 1.
DT   04-NOV-2008, entry version 11.
DE   RecName: Full=Aerobic magnesium-protoporphyrin IX monomethyl ester [oxidative] cyclase;
DE            Short=Aerobic Mg-protoporphyrin IX monomethyl ester oxidative cyclase;
DE            EC=1.14.13.81;
GN   Name=acsF; OrderedLocusNames=BRADO1649;
OS   Bradyrhizobium sp. (strain ORS278).
OC   Bacteria; Proteobacteria; Alphaproteobacteria; Rhizobiales;
OC   Bradyrhizobiaceae; Bradyrhizobium.
OX   NCBI_TaxID=114615;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX   PubMed=17540897; DOI=10.1126/science.1139548;
RA   Giraud E., Moulin L., Vallenet D., Barbe V., Cytryn E., Avarre J.-C.,
RA   Jaubert M., Simon D., Cartieaux F., Prin Y., Bena G., Hannibal L.,
RA   Fardoux J., Kojadinovic M., Vuillet L., Lajus A., Cruveiller S.,
RA   Rouy Z., Mangenot S., Segurens B., Dossat C., Franck W.L.,
RA   Chang W.-S., Saunders E., Bruce D., Richardson P., Normand P.,
RA   Dreyfus B., Pignol D., Stacey G., Emerich D., Vermeglio A.,
RA   Medigue C., Sadowsky M.;
RT   "Legumes symbioses: absence of nod genes in photosynthetic
RT   bradyrhizobia.";
RL   Science 316:1307-1312(2007).
CC   -!- FUNCTION: Catalyzes the formation of the isocyclic ring in
CC       chlorophyll biosynthesis. Mediates the cyclase reaction, which
CC       results in the formation of divinylprotochlorophyllide (Pchlide)
CC       characteristic of all chlorophylls from magnesium-protoporphyrin
CC       IX 13-monomethyl ester (MgPMME) (By similarity).
CC   -!- CATALYTIC ACTIVITY: Magnesium-protoporphyrin IX 13-monomethyl
CC       ester + NADPH + O(2) = 13(1)-hydroxy-magnesium-protoporphyrin IX
CC       13-monomethyl ester + NADP(+) + H(2)O.
CC   -!- CATALYTIC ACTIVITY: 13(1)-hydroxy-magnesium-protoporphyrin IX 13-
CC       monomethyl ester + NADPH + O(2) = 13(1)-oxo-magnesium-
CC       protoporphyrin IX 13-monomethyl ester + NADP(+) + 2 H(2)O.
CC   -!- CATALYTIC ACTIVITY: 13(1)-oxo-magnesium-protoporphyrin IX 13-
CC       monomethyl ester + NADPH + O(2) = divinylprotochlorophyllide +
CC       NADP(+) + 2 H(2)O.
CC   -!- COFACTOR: Iron (By similarity).
CC   -!- PATHWAY: Porphyrin biosynthesis; bacteriochlorophyll biosynthesis
CC       (light-independent).
CC   -!- SIMILARITY: Belongs to the acsF family.
CC   -----------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution-NoDerivs License
CC   -----------------------------------------------------------------------
DR   EMBL; CU234118; CAL75527.1; -; Genomic_DNA.
DR   RefSeq; YP_001203764.1; -.
DR   GeneID; 5118406; -.
DR   GenomeReviews; CU234118_GR; BRADO1649.
DR   KEGG; bra:BRADO1649; -.
DR   GO; GO:0005506; F:iron ion binding; IEA:HAMAP.
DR   GO; GO:0048529; F:magnesium-protoporphyrin IX monomethyl este...; IEA:HAMAP.
DR   GO; GO:0030494; P:bacteriochlorophyll biosynthetic process; IEA:UniProtKB-KW.
DR   GO; GO:0055114; P:oxidation reduction; IEA:UniProtKB-KW.
DR   GO; GO:0015979; P:photosynthesis; IEA:HAMAP.
DR   HAMAP; MF_01840; -; 1.
DR   InterPro; IPR008434; AcsF.
DR   InterPro; IPR003251; Rubrerythrin.
DR   Pfam; PF02915; Rubrerythrin; 1.
DR   TIGRFAMs; TIGR02029; AcsF; 1.
PE   3: Inferred from homology;
KW   Bacteriochlorophyll biosynthesis; Chlorophyll biosynthesis;
KW   Complete proteome; Iron; Metal-binding; NADP; Oxidoreductase;
KW   Photosynthesis.
FT   CHAIN         1    353       Aerobic magnesium-protoporphyrin IX
FT                                monomethyl ester [oxidative] cyclase.
FT                                /FTId=PRO_1000070544.
SQ   SEQUENCE   353 AA;  41481 MW;  F8A5BDD72FE1C2A7 CRC64;
     MIAMEGGSGN ISTKMALEDT ILTPRFYTTD FAAMDRLNVD LVRREWDAVM AELRADHNRK
     HFVRTPEFDK DLNELPEALR AEFKDFLVSS LTAEFSGCVL YAEIKKRITN PDIRELFQFM
     SRDEARHAGF INEILKDHGI GVDLGFLTKT KKYTYFKPKF IFYATYLSEK IGYARYITIY
     RQMERHPERQ FHPIFKWFER WCNDEFRHGE AFALLMRADP KLLSGYNKLW IKFFLLAVFA
     TMHVRDHMRP AFYEALGMPP DEYDMRVFRI TSEISRQVFP VMLDIDNPRF WEGLKRLREI
     SEAIAEAKAQ GGIMGTLKRA VLPLKAALTF GRLYMLPAKN NELPREIRLQ PAW
//