FUNCTION: Catalyzes the formation of the isocyclic ring in chlorophyll biosynthesis. Mediates the cyclase reaction, which results in the formation of divinylprotochlorophyllide (Pchlide) characteristic of all chlorophylls from magnesium-protoporphyrin IX 13-monomethyl ester (MgPMME) (By similarity).
CATALYTIC ACTIVITY: Magnesium-protoporphyrin IX 13-monomethyl ester + NADPH + O₂ = 13¹-hydroxy-magnesium-protoporphyrin IX 13-monomethyl ester + NADP⁺ + H₂O.
CATALYTIC ACTIVITY: 13¹-hydroxy-magnesium-protoporphyrin IX 13-monomethyl ester + NADPH + O₂ = 13¹-oxo-magnesium-protoporphyrin IX 13-monomethyl ester + NADP⁺ + 2 H₂O.
CATALYTIC ACTIVITY: 13¹-oxo-magnesium-protoporphyrin IX 13-monomethyl ester + NADPH + O₂ = divinylprotochlorophyllide + NADP⁺ + 2 H₂O.
COFACTOR: Iron (By similarity).
PATHWAY: Porphyrin metabolism; bacteriochlorophyll biosynthesis (light-independent).
SIMILARITY: Belongs to the acsF family.

Copyright

Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.

Cross-references

Sequence databases

EMBL

CU234118; CAL75527.1; -; Genomic_DNA.

[EMBL / GenBank / DDBJ] [CoDingSequence]

RefSeq

YP_001203764.1; -.

3D structure databases

ModBase

A4YNQ1.

Ontologies

GO:0005506;	Molecular function: iron ion binding (inferred from electronic annotation from HAMAP).
GO:0048529;	Molecular function: magnesium-protoporphyrin IX monomethyl ester (oxidative) cyclase activity (inferred from electronic annotation from HAMAP).
GO:0015979;	Biological process: photosynthesis (inferred from electronic annotation from HAMAP).
QuickGo view.

Family and domain databases

HAMAP

MF_01840; -; 1.
PBIL [Tree]

InterPro

IPR008434; AcsF.
IPR003251; Rubrerythrin.
Graphical view of domain structure.

Pfam

PF02915; Rubrerythrin; 1.
Pfam graphical view of domain structure.

TIGRFAMs

TIGR02029; AcsF; 1.

BLOCKS

A4YNQ1.

Genome annotation databases

GeneID

5118406; -.

GenomeReviews

CU234118_GR; BRADO1649.

KEGG

bra:BRADO1649; -.

CMR

A4YNQ1; BRADO1649.

Other

ProtoNet

A4YNQ1.

UniRef

View cluster of proteins with at least 50% / 90% / 100% identity.

Keywords

Bacteriochlorophyll biosynthesis; Chlorophyll biosynthesis; Complete proteome; Iron; Metal-binding; NADP; Oxidoreductase; Photosynthesis.

Features

Feature table viewer

`Key`	`From To`	`Length`		`Description`	`FTId`
`CHAIN`	`1 353`	`353`		`Aerobic magnesium-protoporphyrin IX monomethyl ester [oxidative] cyclase.`	`PRO_1000070544`

Sequence information

Length: 353 AA [This is the length of the unprocessed precursor]

Molecular weight: 41481 Da [This is the MW of the unprocessed precursor]

CRC64: F8A5BDD72FE1C2A7 [This is a checksum on the sequence]

        10         20         30         40         50         60 
MIAMEGGSGN ISTKMALEDT ILTPRFYTTD FAAMDRLNVD LVRREWDAVM AELRADHNRK 

        70         80         90        100        110        120 
HFVRTPEFDK DLNELPEALR AEFKDFLVSS LTAEFSGCVL YAEIKKRITN PDIRELFQFM 

       130        140        150        160        170        180 
SRDEARHAGF INEILKDHGI GVDLGFLTKT KKYTYFKPKF IFYATYLSEK IGYARYITIY 

       190        200        210        220        230        240 
RQMERHPERQ FHPIFKWFER WCNDEFRHGE AFALLMRADP KLLSGYNKLW IKFFLLAVFA 

       250        260        270        280        290        300 
TMHVRDHMRP AFYEALGMPP DEYDMRVFRI TSEISRQVFP VMLDIDNPRF WEGLKRLREI 

       310        320        330        340        350 
SEAIAEAKAQ GGIMGTLKRA VLPLKAALTF GRLYMLPAKN NELPREIRLQ PAW

A4YNQ1 in FASTA format

View entry in original UniProtKB/Swiss-Prot format
View entry in raw text format (no links)
Report form for errors/updates in this UniProtKB/Swiss-Prot entry

	BLAST submission on ExPASy/SIB or at NCBI (USA)		Sequence analysis tools: ProtParam, ProtScale, Compute pI/Mw, PeptideMass, PeptideCutter, Dotlet (Java)
	ScanProsite, MotifScan		Submit a homology modeling request to SWISS-MODEL
	NPSA Sequence analysis tools