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UniProtKB/Swiss-Prot entry A4Y3I1

[Entry info] [Name and origin] [References] [Comments] [Cross-references] [Keywords] [Features] [Sequence] [Tools]

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Entry information
Entry name MAO1_SHEPC
Primary accession number A4Y3I1
Secondary accession numbers None
Integrated into Swiss-Prot on February 5, 2008
Sequence was last modified on May 29, 2007 (Sequence version 1)
Annotations were last modified on    November 25, 2008 (Entry version 15)
Name and origin of the protein
Protein name NAD-dependent malic enzyme
Synonyms NAD-ME
EC 1.1.1.38
Gene name
Name: sfcA
OrderedLocusNames: Sputcn32_0784
From
Shewanella putrefaciens (strain CN-32 / ATCC BAA-453) [TaxID: 319224] [HAMAP proteome]
Taxonomy Bacteria; Proteobacteria; Gammaproteobacteria; Alteromonadales; Shewanellaceae; Shewanella.
Protein existence 3: Inferred from homology;
References
[1]
 NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina del Rio T., Hammon N., Israni S., Dalin E., Tice H., Pitluck S., Chain P., Malfatti S., Shin M., Vergez L., Schmutz J., Larimer F., Land M., Hauser L., Kyrpides N., Mikhailova N., Romine M.F., Fredrickson J., Tiedje J., Richardson P.;
"Complete sequence of Shewanella putrefaciens CN-32.";
Submitted (APR-2007) to the EMBL/GenBank/DDBJ databases.
Comments
Copyright
Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.
Cross-references
Sequence databases
EMBL
CP000681; ABP74514.1; -; Genomic_DNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
RefSeq YP_001182313.1; -.
3D structure databases
ModBase A4Y3I1.
Ontologies
GO
GO:0016619; Molecular function: malate dehydrogenase (oxaloacetate-decarboxylating) activity (inferred from electronic annotation from HAMAP).
GO:0046872; Molecular function: metal ion binding (inferred from electronic annotation from UniProtKB-KW).
GO:0051287; Molecular function: NAD binding (inferred from electronic annotation from InterPro).
GO:0006108; Biological process: malate metabolic process (inferred from electronic annotation from InterPro).
GO:0055114; Biological process: oxidation reduction (inferred from electronic annotation from UniProtKB-KW).
QuickGo view.
Family and domain databases
HAMAP MF_01619; -; 1.
PBIL [Tree]
InterPro IPR015884; Malic_enzyme_CS.
IPR012301; Malic_N.
IPR012302; Malic_NAD_bd.
IPR001891; Malic_OxRdtase.
IPR016040; NAD(P)-bd.
Graphical view of domain structure.
Gene3D G3DSA:3.40.50.720; NAD(P)-bd; 1.
Pfam PF00390; malic; 1.
PF03949; Malic_M; 1.
Pfam graphical view of domain structure.
PRINTS PR00072; MALOXRDTASE.
PROSITE PS00331; MALIC_ENZYMES; 1.
BLOCKS A4Y3I1.
ProtoNet A4Y3I1.
Genome annotation databases
GeneID 5081994; -.
GenomeReviews CP000681_GR; Sputcn32_0784.
KEGG spc:Sputcn32_0784; -.
CMR A4Y3I1; Sputcn32_0784.
Other
UniRef View cluster of proteins with at least 50% / 90% / 100% identity.
Keywords
Complete proteome; Metal-binding; NAD; Oxidoreductase.
Features
SEVIEWER logo Feature table viewer
KeyFrom   To Length Description FTId
CHAIN   1   562  562     NAD-dependent malic enzyme. PRO_1000069544
ACT_SITE   101   101        Proton donor (By similarity). 
ACT_SITE   172   172        Proton acceptor (By similarity). 
METAL   243   243        Divalent metal cation (By similarity). 
METAL   244   244        Divalent metal cation (By similarity). 
METAL   267   267        Divalent metal cation (By similarity). 
BINDING   154   154        NAD (By similarity). 
BINDING   267   267        NAD (By similarity). 
BINDING   415   415        NAD (By similarity). 
SITE   267   267  1     Important for activity (By similarity). 
Sequence information
Length: 562 AA [This is the length of the unprocessed precursor] Molecular weight: 62188 Da [This is the MW of the unprocessed precursor] CRC64: D7439DDEC6262DC8 [This is a checksum on the sequence] 
        10         20         30         40         50         60 
MDDNKRPLYL PFAGPAILEA PLINKGSAFS EEERIFFNLE GLVPYAIETI EEQASRAYDQ 

        70         80         90        100        110        120 
FRSFNNDLDK HIYLRNIQDT NETLFYRLVQ NNISEMMPII YTPTVGLACE RFSKNYRRNR 

       130        140        150        160        170        180 
GLFISYPNKD RIDDILNNST RQKVKIIVVT DGERILGLGD QGIGGMGIPI GKLSLYTSCG 

       190        200        210        220        230        240 
GISPAYTLPI TLDVGTDNPQ LLEDPMYMGW RHPRIGGEEY AEFIEAFMQA VHVRWPDTLI 

       250        260        270        280        290        300 
QFEDFAQKNA MPILERYKDR YCCFNDDIQG TAAVAVGCLL AACKAAGTEL NQQRIAFLGA 

       310        320        330        340        350        360 
GSAGCGIAEA IVAQMVSEGI SDEQARTQVC MVDRWGLLLD NMPNLLPFQQ KLAQKCANIQ 

       370        380        390        400        410        420 
NWNNFSDNIS LLDVVNNAKP TVLIGVSGVP GLFTEEIIRA MHSHCARPII FPMSNPTSRV 

       430        440        450        460        470        480 
EATPKDILHW TSGQALVATG SPFEPVVVDG ETYEIAQCNN SFIFPGIGLG VLASGARHVS 

       490        500        510        520        530        540 
DAMLMASSRA LAECSPLAID GTGPLLPKLE DIHAVSKHIA FAVGKVAVEQ GLTLPMSDEI 

       550        560 
LQQSIEGNFW KPEYRRYKRT SF
A4Y3I1 in FASTA format

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