NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Reith M.E., Singh R.K., Curtis B., Boyd J., Bouevitch A., Kimball J., Munholland J., Murphy C., Sarty D., Williams J., Nash J., Johnson S., Brown L.;
"The genome sequence of Aeromonas salmonicida subsp. salmonicida A449.";
Submitted (MAR-2007) to the EMBL/GenBank/DDBJ databases.

Comments

CATALYTIC ACTIVITY: (S)-dihydroorotate + O₂ = orotate + H₂O₂.
COFACTOR: Binds 1 FMN per subunit (By similarity).
PATHWAY: Pyrimidine metabolism; UMP biosynthesis via de novo pathway; UMP from HCO(3)(-): step 4/6 .
SUBUNIT: Homodimer (By similarity).
SUBCELLULAR LOCATION: Cell membrane; Peripheral membrane protein (By similarity).
SIMILARITY: Belongs to the dihydroorotate dehydrogenase family. Type 2 subfamily.

Copyright

Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.

Cross-references

Sequence databases

EMBL

CP000644; ABO90064.1; -; Genomic_DNA.

[EMBL / GenBank / DDBJ] [CoDingSequence]

RefSeq

YP_001141812.1; -.

3D structure databases

ModBase

A4SME2.

Ontologies

GO:0005886;	Cellular component: plasma membrane (inferred from electronic annotation from HAMAP).
GO:0004158;	Molecular function: dihydroorotate oxidase activity (inferred from electronic annotation from HAMAP).
QuickGo view.

Family and domain databases

HAMAP

MF_00225; -; 1.
PBIL [Tree]

InterPro

IPR012135; DHO_DHase_1_2.
IPR005719; DHO_DHase_2.
IPR001295; Dihydroorotate_DHase_core.
Graphical view of domain structure.

Pfam

PF01180; DHO_dh; 1.
Pfam graphical view of domain structure.

PIRSF

PIRSF000164; DHO_oxidase; 1.

TIGRFAMs

TIGR01036; pyrD_sub2; 1.

PROSITE

PS00911; DHODEHASE_1; 1.
PS00912; DHODEHASE_2; 1.

BLOCKS

A4SME2.

Genome annotation databases

GeneID

4996284; -.

GenomeReviews

CP000644_GR; ASA_1993.

KEGG

asa:ASA_1993; -.

CMR

A4SME2; ASA_1993.

Other

ProtoNet

A4SME2.

UniRef

View cluster of proteins with at least 50% / 90% / 100% identity.

Keywords

Cell membrane; Complete proteome; Flavoprotein; FMN; Membrane; Oxidoreductase; Pyrimidine biosynthesis.

Features

Feature table viewer

`Key`	`From To`	`Length`	`Description`	`FTId`
`CHAIN`	`1 336`	`336`	`Dihydroorotate dehydrogenase.`	`PRO_1000024148`
`ACT_SITE`	`175 175`		`Nucleophile (By similarity).`

Sequence information

Length: 336 AA [This is the length of the unprocessed precursor]

Molecular weight: 36411 Da [This is the MW of the unprocessed precursor]

CRC64: EC003C32D9230911 [This is a checksum on the sequence]

        10         20         30         40         50         60 
MLYPLARHFL FKFNPEQAHD LSIKYLPRLL GTPLDCFFRH SLPKRPVTVM GLYFANPVGL 

        70         80         90        100        110        120 
AAGLDKDGEC IDAFGAMGFG FIEVGTVTPK PQSGNDKPRL FRVIPAEGII NRMGFNNKGV 

       130        140        150        160        170        180 
DHLVAQVKKA KYQGVIGINI GKNKDTPIEQ GKDDYLICMD KVYDHAGYIA VNISSPNTPG 

       190        200        210        220        230        240 
LRSLQYGDAL DELLAALKVR QQELAAQYKK YVPLAVKIAP DLSLDEINQV AASLIKNGID 

       250        260        270        280        290        300 
GVIATNTTLD REMIYDMPHA GEAGGLSGRP LQHKSTEVIR QLAKALDGAL PIIGVGGIDS 

       310        320        330 
AMAAREKLAA GASLVQIYSG FIYKGPSLVK EIVTHI

A4SME2 in FASTA format

View entry in original UniProtKB/Swiss-Prot format
View entry in raw text format (no links)
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	BLAST submission on ExPASy/SIB or at NCBI (USA)		Sequence analysis tools: ProtParam, ProtScale, Compute pI/Mw, PeptideMass, PeptideCutter, Dotlet (Java)
	ScanProsite, MotifScan		Submit a homology modeling request to SWISS-MODEL
	NPSA Sequence analysis tools