NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina del Rio T., Hammon N., Israni S., Dalin E., Tice H., Pitluck S., Chain P., Malfatti S., Shin M., Vergez L., Schmutz J., Larimer F., Land M., Hauser L., Kyrpides N., Tiedje J., Richardson P.;
"Complete sequence of chromosome 1 of Burkholderia vietnamiensis G4.";
Submitted (MAR-2007) to the EMBL/GenBank/DDBJ databases.

Comments

FUNCTION: Catalyzes the NADPH-dependent reduction of 7-cyano-7-deazaguanine (preQ0) to 7-aminomethyl-7-deazaguanine (preQ1) (By similarity).
CATALYTIC ACTIVITY: 7-aminomethyl-7-carbaguanine + 2 NADP⁺ = 7-cyano-7-carbaguanine + 2 NADPH.
PATHWAY: tRNA modification; queuosine-tRNA biosynthesis .
SUBCELLULAR LOCATION: Cytoplasm (Probable).
SIMILARITY: Belongs to the GTP cyclohydrolase I family. QueF type 2 subfamily.

Copyright

Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.

Cross-references

Sequence databases

EMBL

CP000614; ABO55834.1; -; Genomic_DNA.

[EMBL / GenBank / DDBJ] [CoDingSequence]

RefSeq

YP_001120669.1; -.

3D structure databases

ModBase

A4JHT1.

Ontologies

GO:0005737;	Cellular component: cytoplasm (inferred from electronic annotation from HAMAP).
GO:0046857;	Molecular function: oxidoreductase activity, acting on other nitrogenous compounds as donors, with NAD or NADP as acceptor (inferred from electronic annotation from HAMAP).
GO:0008616;	Biological process: queuosine biosynthetic process (inferred from electronic annotation from HAMAP).
QuickGo view.

Family and domain databases

HAMAP

MF_00817; -; 1.
PBIL [Tree]

InterPro

IPR016428; CN_OxRdtase_NADPH-dep_YqcD.
IPR001474; GTP_CycOHase_I.
Graphical view of domain structure.

Pfam

PF01227; GTP_cyclohydroI; 1.
Pfam graphical view of domain structure.

PIRSF

PIRSF004750; Nitrile_oxidored_YqcD_prd; 1.

TIGRFAMs

TIGR03138; QueF; 1.

BLOCKS

A4JHT1.

Genome annotation databases

GeneID

4954588; -.

GenomeReviews

CP000614_GR; Bcep1808_2843.

KEGG

bvi:Bcep1808_2843; -.

CMR

A4JHT1; Bcep1808_2843.

Other

ProtoNet

A4JHT1.

UniRef

View cluster of proteins with at least 50% / 90% / 100% identity.

Keywords

Complete proteome; Cytoplasm; NADP; Oxidoreductase; Queuosine biosynthesis.

Features

Feature table viewer

`Key`	`From To`	`Length`		`Description`	`FTId`
`CHAIN`	`1 274`	`274`		`NADPH-dependent 7-cyano-7-deazaguanine reductase.`	`PRO_1000062335`

Sequence information

Length: 274 AA [This is the length of the unprocessed precursor]

Molecular weight: 30291 Da [This is the MW of the unprocessed precursor]

CRC64: 0ED5A700F3AF5B80 [This is a checksum on the sequence]

        10         20         30         40         50         60 
MNPEHSPLGK ATVYAAQYDA SLLFPIPRAG AREQLGIAAA LPFFGTDIWN AYELSWLNAR 

        70         80         90        100        110        120 
GKPQLAVATF YVPAESPNIV ESKSFKLYLG SFAQSKFDSV DAVRDVLKRD VSAACGASVS 

       130        140        150        160        170        180 
VQLVSPHDFG KLQMEELDGL SLDRLDLDTD VYEPDPSLLS AAADEAPVEE TLVSDLLRSN 

       190        200        210        220        230        240 
CPVTGQPDWG SVQIHYVGPQ IDHAGLLRYI ISFRNHTGFH EQCVERIFLD ILQACKPLKL 

       250        260        270 
AVYARYTRRG GLDINPFRTN YNQSMPDNAR TARQ

A4JHT1 in FASTA format

View entry in original UniProtKB/Swiss-Prot format
View entry in raw text format (no links)
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	BLAST submission on ExPASy/SIB or at NCBI (USA)		Sequence analysis tools: ProtParam, ProtScale, Compute pI/Mw, PeptideMass, PeptideCutter, Dotlet (Java)
	ScanProsite, MotifScan		Submit a homology modeling request to SWISS-MODEL
	NPSA Sequence analysis tools