[1]	NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. TISSUE=Kidney; DOI=10.1101/gr.2596504; PubMed=15489334 [NCBI, ExPASy, EBI, Israel, Japan] The MGC Project Team; "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."; Genome Res. 14:2121-2127(2004).

Comments

FUNCTION: Bifunctional enzyme that catalyzes the first two steps in lysine degradation. The N-terminal and the C-terminal contain lysine-oxoglutarate reductase and saccharopine dehydrogenase activity, respectively (By similarity).
CATALYTIC ACTIVITY: N⁶-(L-1,3-dicarboxypropyl)-L-lysine + NADP⁺ + H₂O = L-lysine + 2-oxoglutarate + NADPH.
CATALYTIC ACTIVITY: N⁶-(L-1,3-dicarboxypropyl)-L-lysine + NAD⁺ + H₂O = L-glutamate + 2-aminoadipate 6-semialdehyde + NADH.
PATHWAY: Amino-acid degradation; L-lysine degradation via saccharopine pathway; glutaryl-CoA from L-lysine: step 1/6 .
PATHWAY: Amino-acid degradation; L-lysine degradation via saccharopine pathway; glutaryl-CoA from L-lysine: step 2/6 .
SUBUNIT: Homodimer (By similarity).
SUBCELLULAR LOCATION: Mitochondrion (By similarity).
SIMILARITY: In the N-terminal section; belongs to the AlaDH/PNT family.
SIMILARITY: In the C-terminal section; belongs to the saccharopine dehydrogenase family.

Copyright

Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.

Cross-references

Sequence databases

EMBL

BC128771; AAI28772.1; -; mRNA.

[EMBL / GenBank / DDBJ] [CoDingSequence]

RefSeq

NP_001094433.1; -.

UniGene

Rn.198671

3D structure databases

ModBase

A2VCW9.

Organism-specific databases

RGD

1310811; Aass.

Ontologies

GO:0005739;	Cellular component: mitochondrion (inferred from electronic annotation from UniProtKB-KW).
GO:0005488;	Molecular function: binding (inferred from electronic annotation from InterPro).
GO:0009055;	Molecular function: electron carrier activity (inferred from electronic annotation from InterPro).
GO:0047131;	Molecular function: saccharopine dehydrogenase (NAD+, L-glutamate-forming) activity (inferred from electronic annotation from EC).
GO:0047130;	Molecular function: saccharopine dehydrogenase (NADP+, L-lysine-forming) activity (inferred from electronic annotation from EC).
GO:0055114;	Biological process: oxidation reduction (inferred from electronic annotation from UniProtKB-KW).
QuickGo view.

Family and domain databases

InterPro

IPR007698; Ala_DHase/PNT_C.
IPR008142; Ala_DHase/PNT_CS1.
IPR008143; Ala_DHase/PNT_CS2.
IPR007886; Ala_DHase/PNT_N.
IPR005097; Saccharopine_DHase.
Graphical view of domain structure.

Pfam

PF01262; AlaDh_PNT_C; 1.
PF05222; AlaDh_PNT_N; 1.
PF03435; Saccharop_dh; 1.
Pfam graphical view of domain structure.

PROSITE

PS00836; ALADH_PNT_1; FALSE_NEG.
PS00837; ALADH_PNT_2; FALSE_NEG.

ProtoNet

A2VCW9.

Genome annotation databases

GeneID

296925; -.

KEGG

rno:296925; -.

Phylogenomic databases

HOVERGEN

A2VCW9; -.

Other

NextBio

641904; -.

UniRef

View cluster of proteins with at least 50% / 90% / 100% identity.

Keywords

Mitochondrion; Multifunctional enzyme; NAD; NADP; Oxidoreductase; Transit peptide.

Features

Feature table viewer

Feature aligner

`Key`	`From To`	`Length`	`Description`	`FTId`
`TRANSIT`	`1 32`	`32`	`Mitochondrion (By similarity).`
`CHAIN`	`33 926`	`894`	`Alpha-aminoadipic semialdehyde synthase, mitochondrial.`	`PRO_0000315868`
`REGION`	`33 455`	`423`	`Lysine-ketoglutarate reductase (By similarity).`
`REGION`	`477 926`	`450`	`Saccharopine dehydrogenase (By similarity).`

Sequence information

Length: 926 AA [This is the length of the unprocessed precursor]

Molecular weight: 102908 Da [This is the MW of the unprocessed precursor]

CRC64: 4350DDB240B4075F [This is a checksum on the sequence]

        10         20         30         40         50         60 
MLRAQRLRLA RLRACVSRGL HHKPVMALRR EDVNAWERRA PLAPKHIKGI TKLGYKVLIQ 

        70         80         90        100        110        120 
PSNRRAIHDK EYVRAGGILQ EDITEACLIL GVKRPPEEKL MSKKTYAFFS HTIKAQEANM 

       130        140        150        160        170        180 
SLLDEVLKQE IRLIDYEKMV DHRGSRIVAF GHWAGVAGMI NILHGMGLRL LALGHHTPFM 

       190        200        210        220        230        240 
HLGMAHNYRN SSQAVQAVRD AGYEISLGLM PKSIGPLTFV FTGTGNVSKG AQEVFNELPC 

       250        260        270        280        290        300 
EYVEPHELKE VSKTGDLRKV YGTVLSRHHH LVRKTDGVYD PVEYEKYPER YISRFNADIA 

       310        320        330        340        350        360 
PYTTCLINGI YWEQNTPRLL TRQDAQSLLV PVKSSVVPVE GCPELPHKLV AICDISADTG 

       370        380        390        400        410        420 
GSIDFMTECT TIERPFCMYD ADQHIIHDSV EGSGILMCSI DNLPAQLPIE ATEYFGDMLY 

       430        440        450        460        470        480 
PYVEEMLLSD ASQPLESQNF SPVVRDAVIT SNGLLTDKYK YIQKLRESRE RIQFLSMSTK 

       490        500        510        520        530        540 
KKVLVLGSGY VSGPVLEYLS RGNNIEITLG SDMTNQMQQL SKKYDINTVN VTVGKQEDKL 

       550        560        570        580        590        600 
QSLVESQDLV ISLLPYVLHP VVAKACIDSK VNMVTASYIT PAMKELEKSV DDAGITVIGE 

       610        620        630        640        650        660 
LGLDPGLDHM LAMETIDKAK DLGATIESYV SYCGGLPAPE HSDNPLRYKF SWSPVGVLMN 

       670        680        690        700        710        720 
IMQPASYLLN GKVVNVTGGV SFLNSVTPMD YFPGLNLEGY PNRDSTKYAE IYGISSAHTL 

       730        740        750        760        770        780 
LRGTLRYKGY SKALNGFVKL GLINRETYPA LRPEANPLTW KQLLCDLVGI SRSSSCEKLK 

       790        800        810        820        830        840 
EVVFTKLGGD STQLEAAEWL GLLGDEQVPQ AESIVDAFSK HLVSKLSYGP EEKDMIVMRD 

       850        860        870        880        890        900 
SFGIRHPSGH LENKTIDLVV YGDFNGFSAM AKTVGLPTAM AAKMLLDGEI ETKGLMGPFS 

       910        920 
KEIYGPILER IKAEGIVFNT QSTIKL

A2VCW9 in FASTA format

View entry in original UniProtKB/Swiss-Prot format
View entry in raw text format (no links)
Report form for errors/updates in this UniProtKB/Swiss-Prot entry

	BLAST submission on ExPASy/SIB or at NCBI (USA)		Sequence analysis tools: ProtParam, ProtScale, Compute pI/Mw, PeptideMass, PeptideCutter, Dotlet (Java)
	ScanProsite, MotifScan		Submit a homology modeling request to SWISS-MODEL
	NPSA Sequence analysis tools