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UniProtKB/Swiss-Prot entry A2RIA3

[Entry info] [Name and origin] [References] [Comments] [Cross-references] [Keywords] [Features] [Sequence] [Tools]

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Entry information
Entry name LDH2_LACLM
Primary accession number A2RIA3
Secondary accession number Q7WYP6
Integrated into Swiss-Prot on May 1, 2007
Sequence was last modified on March 6, 2007 (Sequence version 1)
Annotations were last modified on    July 22, 2008 (Entry version 15)
Name and origin of the protein
Protein name L-lactate dehydrogenase 2
Synonyms L-LDH 2
EC 1.1.1.27
Gene name
Name: ldhB
OrderedLocusNames: llmg_0392
From
Lactococcus lactis subsp. cremoris (strain MG1363) [TaxID: 416870] [HAMAP proteome]
Taxonomy Bacteria; Firmicutes; Lactobacillales; Streptococcaceae; Lactococcus.
Protein existence 1: Evidence at protein level;
References
[1]
 NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND CATALYTIC ACTIVITY.
STRAIN=MG1363 / NZ9000;
DOI=10.1128/JB.185.15.4499-4507.2003; PubMed=12867459 [NCBI, ExPASy, EBI, Israel, Japan]
Bongers R.S., Hoefnagel M.H.N., Starrenburg M.J.C., Siemerink M.A.J., Arends J.G.A., Hugenholtz J., Kleerebezem M.;
"IS981-mediated adaptive evolution recovers lactate production by ldhB transcription activation in a lactate dehydrogenase-deficient strain of Lactococcus lactis.";
J. Bacteriol. 185:4499-4507(2003).
[2]
 NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
DOI=10.1128/JB.01768-06; PubMed=17307855 [NCBI, ExPASy, EBI, Israel, Japan]
Wegmann U., O'Connell-Motherway M., Zomer A., Buist G., Shearman C., Canchaya C., Ventura M., Goesmann A., Gasson M.J., Kuipers O.P., van Sinderen D., Kok J.;
"The complete genome sequence of the lactic acid bacterial paradigm Lactococcus lactis subsp. cremoris MG1363.";
J. Bacteriol. 189:3256-3270(2007).
Comments
Copyright
Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.
Cross-references
Sequence databases
EMBL
AY230155; AAO60056.1; -; Genomic_DNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
AM406671; CAL96997.1; -; Genomic_DNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
RefSeq YP_001031744.1; -.
3D structure databases
ModBase A2RIA3.
Ontologies
GO
GO:0005737; Cellular component: cytoplasm (inferred from electronic annotation from HAMAP).
GO:0004459; Molecular function: L-lactate dehydrogenase activity (inferred from electronic annotation from HAMAP).
GO:0019642; Biological process: anaerobic glycolysis (inferred from electronic annotation from HAMAP).
QuickGo view.
Family and domain databases
HAMAP MF_00488; -; 1.
PBIL [Tree]
InterPro IPR001557; L-lactate/malate_DHase.
IPR011304; L-lactate_DHase.
IPR001236; Lactate/malate_DHase.
IPR015955; Lactate_DHase/Glyco_Ohase_4_C.
IPR016040; NAD(P)-bd.
Graphical view of domain structure.
Gene3D G3DSA:3.90.110.10; lact_mal_DH; 1.
G3DSA:3.40.50.720; NAD(P)-bd; 1.
Pfam PF02866; Ldh_1_C; 1.
PF00056; Ldh_1_N; 1.
Pfam graphical view of domain structure.
PIRSF PIRSF000102; Lac_mal_DH; 1.
PRINTS PR00086; LLDHDRGNASE.
TIGRFAMs TIGR01771; L-LDH-NAD; 1.
PROSITE PS00064; L_LDH; 1.
BLOCKS A2RIA3.
Genome annotation databases
GeneID 4797254; -.
GenomeReviews AM406671_GR; llmg_0392.
KEGG llm:llmg_0392; -.
CMR A2RIA3; llmg_0392.
Other
ProtoNet A2RIA3.
UniRef View cluster of proteins with at least 50% / 90% / 100% identity.
Keywords
Complete proteome; Cytoplasm; Glycolysis; NAD; Oxidoreductase; Phosphoprotein.
Features
SEVIEWER logo Feature table viewer
KeyFrom   To Length Description FTId
CHAIN   1   314  314     L-lactate dehydrogenase 2. PRO_0000285235
NP_BIND   14    42  29     NAD (By similarity). 
ACT_SITE   178   178        Proton acceptor (By similarity). 
BINDING   91    91        Substrate (By similarity). 
BINDING   123   123        NAD or substrate (By similarity). 
BINDING   154   154        Substrate (By similarity). 
BINDING   232   232        Substrate (By similarity). 
MOD_RES   223   223        Phosphotyrosine (By similarity). 
Sequence information
Length: 314 AA [This is the length of the unprocessed precursor] Molecular weight: 34398 Da [This is the MW of the unprocessed precursor] CRC64: 9D5114712ACB75A4 [This is a checksum on the sequence] 
        10         20         30         40         50         60 
MKITSRKVVV IGTGFVGTSI AYSMINQGLV NELVLIDVNQ DKAEGEALDL LDGISWAQEN 

        70         80         90        100        110        120 
VIVRAGNYKD CENADIVVIT AGVNQKPGQS RLDLVNTNAK IMRSIVTQVM DSGFDGIFVI 

       130        140        150        160        170        180 
ASNPVDILTY VAWETSGLDQ SRIVGTGTTL DTTRFRKELA TKLEIDPRSV HGYIIGEHGD 

       190        200        210        220        230        240 
SEVAVWSHTT IGGKPILEFI VKNKKIGLED LSNLSNKVKN AAYEIIDKKQ ATYYGIGMST 

       250        260        270        280        290        300 
ARIVKAILNN EQVILPVSAY LRGEYGQEGV FTGVPSVVNQ NGVREIIELN IDAYEMKQFE 

       310 
KSVSQLKEVI ESIK
A2RIA3 in FASTA format

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