NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
STRAIN=SAG 48.80;
DOI=10.1186/1741-7007-5-2; PubMed=17222354 [NCBI, ExPASy, EBI, Israel, Japan]
Lemieux C., Otis C., Turmel M.;
"A clade uniting the green algae Mesostigma viride and Chlorokybus atmophyticus represents the deepest branch of the Streptophyta in chloroplast genome-based phylogenies.";
BMC Biol. 5:2-2(2007).

Comments

FUNCTION: The pyruvate dehydrogenase complex catalyzes the overall conversion of pyruvate to acetyl-CoA and CO(2). It contains multiple copies of three enzymatic components: pyruvate dehydrogenase (E1), dihydrolipoamide acetyltransferase (E2) and lipoamide dehydrogenase (E3) (By similarity).
CATALYTIC ACTIVITY: Pyruvate + [dihydrolipoyllysine-residue acetyltransferase] lipoyllysine = [dihydrolipoyllysine-residue acetyltransferase] S-acetyldihydrolipoyllysine + CO₂.
COFACTOR: Thiamine pyrophosphate (By similarity).
SUBUNIT: Heterodimer of an alpha and a beta chain (By similarity).
SUBCELLULAR LOCATION: Plastid, chloroplast.

Copyright

Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.

Cross-references

Sequence databases

EMBL

DQ422812; ABM87959.1; -; Genomic_DNA.

[EMBL / GenBank / DDBJ] [CoDingSequence]

RefSeq

YP_001019115.1; -.

3D structure databases

ModBase

A2CI50.

Family and domain databases

InterPro

IPR005476; Transketo_C.
IPR005475; Transketo_Cen_R.
IPR015941; Transketolase_C-like.
Graphical view of domain structure.

Gene3D

G3DSA:3.40.50.920; Transketo_C_like; 1.

Pfam

PF02779; Transket_pyr; 1.
PF02780; Transketolase_C; 1.
Pfam graphical view of domain structure.

BLOCKS

A2CI50.

Genome annotation databases

GeneID

4783272; -.

Other

ProtoNet

A2CI50.

UniRef

View cluster of proteins with at least 50% / 90% / 100% identity.

Keywords

Chloroplast; Glycolysis; Oxidoreductase; Plastid; Pyruvate; Thiamine pyrophosphate.

Features

Feature table viewer

`Key`	`From To`	`Length`	`Description`	`FTId`
`CHAIN`	`1 335`	`335`	`Pyruvate dehydrogenase E1 component subunit beta.`	`PRO_0000280103`
`BINDING`	`60 60`		`Thiamine pyrophosphate (By similarity).`

Sequence information

Length: 335 AA [This is the length of the unprocessed precursor]

Molecular weight: 36680 Da [This is the MW of the unprocessed precursor]

CRC64: E846941B42AF42AF [This is a checksum on the sequence]

        10         20         30         40         50         60 
MAVRFLFEAL QKAIDEEMER EKRVVLIGED IGHYGGSYKV TQGLYGKYGK HRVIDTPIAE 

        70         80         90        100        110        120 
YSFVGAAVGA AATGLIPVVE GMNMAFILLA YSQISNNMGM LCATSGGHFQ VPMVLRGPGG 

       130        140        150        160        170        180 
IGKQLGAEHS QRLESYFQSV PGLQIVTCST PYNAKGLLKS AIRSKNPILF IEHVLLYNLK 

       190        200        210        220        230        240 
GEVPDNDYLL PLEKAELVRE GSDITVLTYS RQRYNVIQAV KVLVEEGYDP EVIDLISLKP 

       250        260        270        280        290        300 
FDMETIGKSI QKTHKVLIVE ECMMTGGISN VLQSLIIDNF FDALDAAPLI LSSPNVPTPY 

       310        320        330 
TGPLEEATVV QTIDIIESIE YGITGKPPKP RTAKK

A2CI50 in FASTA format

View entry in original UniProtKB/Swiss-Prot format
View entry in raw text format (no links)
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	BLAST submission on ExPASy/SIB or at NCBI (USA)		Sequence analysis tools: ProtParam, ProtScale, Compute pI/Mw, PeptideMass, PeptideCutter, Dotlet (Java)
	ScanProsite, MotifScan		Submit a homology modeling request to SWISS-MODEL
	NPSA Sequence analysis tools