NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina del Rio T., Dalin E., Tice H., Pitluck S., Chain P., Malfatti S., Shin M., Vergez L., Schmutz J., Larimer F., Land M., Hauser L., Kyrpides N., Lykidis A., Walker C., Richardson P.;
"Complete sequence of chromosome 1 of Desulfovibrio vulgaris subsp. vulgaris DP4.";
Submitted (DEC-2006) to the EMBL/GenBank/DDBJ databases.

Comments

FUNCTION: Catalyzes the NADPH-dependent reduction of 7-cyano-7-deazaguanine (preQ0) to 7-aminomethyl-7-deazaguanine (preQ1) (By similarity).
CATALYTIC ACTIVITY: 7-aminomethyl-7-carbaguanine + 2 NADP⁺ = 7-cyano-7-carbaguanine + 2 NADPH.
PATHWAY: tRNA modification; queuosine-tRNA biosynthesis .
SUBCELLULAR LOCATION: Cytoplasm (Probable).
SIMILARITY: Belongs to the GTP cyclohydrolase I family. QueF type 1 subfamily.

Copyright

Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.

Cross-references

Sequence databases

EMBL

CP000527; ABM29041.1; -; Genomic_DNA.

[EMBL / GenBank / DDBJ] [CoDingSequence]

RefSeq

YP_967468.1; -.

3D structure databases

ModBase

A1VF25.

Ontologies

GO:0005737;	Cellular component: cytoplasm (inferred from electronic annotation from HAMAP).
GO:0046857;	Molecular function: oxidoreductase activity, acting on other nitrogenous compounds as donors, with NAD or NADP as acceptor (inferred from electronic annotation from HAMAP).
GO:0008616;	Biological process: queuosine biosynthetic process (inferred from electronic annotation from HAMAP).
QuickGo view.

Family and domain databases

HAMAP

MF_00818; -; 1.
PBIL [Tree]

InterPro

IPR016856; CN_OxRdtase_NADPH-dep_QueF.
IPR001474; GTP_CycOHase_I.
Graphical view of domain structure.

Pfam

PF01227; GTP_cyclohydroI; 1.
Pfam graphical view of domain structure.

PIRSF

PIRSF027377; Nitrile_oxidored_QueF; 1.

TIGRFAMs

TIGR03139; QueF-II; 1.

BLOCKS

A1VF25.

Genome annotation databases

GeneID

4662737; -.

GenomeReviews

CP000527_GR; Dvul_2025.

KEGG

dvl:Dvul_2025; -.

CMR

A1VF25; Dvul_2025.

Other

ProtoNet

A1VF25.

UniRef

View cluster of proteins with at least 50% / 90% / 100% identity.

Keywords

Complete proteome; Cytoplasm; NADP; Oxidoreductase; Queuosine biosynthesis.

Features

Feature table viewer

`Key`	`From To`	`Length`		`Description`	`FTId`
`CHAIN`	`1 165`	`165`		`NADPH-dependent 7-cyano-7-deazaguanine reductase.`	`PRO_1000062383`

Sequence information

Length: 165 AA [This is the length of the unprocessed precursor]

Molecular weight: 18924 Da [This is the MW of the unprocessed precursor]

CRC64: 01B9159B986A01AE [This is a checksum on the sequence]

        10         20         30         40         50         60 
MTTRSTDQTE HLRALGQKTP YPAAGPSTDL LEAFPNRFPD RPYIVSIAFP EFTSLCPVTG 

        70         80         90        100        110        120 
QPDFATIVVE YIPDQFCVES KSFKVYMFAF RDHQSFMETI TNTILDDMTT KLQPLWCRVK 

       130        140        150        160 
GLFTPRGGTQ LHVFAERFKE VEPARAQALR DMVSEWKREN NRHGA

A1VF25 in FASTA format

View entry in original UniProtKB/Swiss-Prot format
View entry in raw text format (no links)
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	BLAST submission on ExPASy/SIB or at NCBI (USA)		Sequence analysis tools: ProtParam, ProtScale, Compute pI/Mw, PeptideMass, PeptideCutter, Dotlet (Java)
	ScanProsite, MotifScan		Submit a homology modeling request to SWISS-MODEL
	NPSA Sequence analysis tools