NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina del Rio T., Hammon N., Israni S., Pitluck S., Goltsman E., Schmutz J., Larimer F., Land M., Hauser L., Mikhailova N., Li T., Overmann J., Bryant D.A., Richardson P.;
"Complete sequence of Chlorobium phaeobacteroides DSM 266.";
Submitted (DEC-2006) to the EMBL/GenBank/DDBJ databases.

Comments

FUNCTION: Catalyzes the NADPH-dependent reduction of 7-cyano-7-deazaguanine (preQ0) to 7-aminomethyl-7-deazaguanine (preQ1) (By similarity).
CATALYTIC ACTIVITY: 7-aminomethyl-7-carbaguanine + 2 NADP⁺ = 7-cyano-7-carbaguanine + 2 NADPH.
PATHWAY: tRNA modification; queuosine-tRNA biosynthesis .
SUBCELLULAR LOCATION: Cytoplasm (Probable).
SIMILARITY: Belongs to the GTP cyclohydrolase I family. QueF type 1 subfamily.

Copyright

Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.

Cross-references

Sequence databases

EMBL

CP000492; ABL64617.1; -; Genomic_DNA.

[EMBL / GenBank / DDBJ] [CoDingSequence]

RefSeq

YP_911041.1; -.

3D structure databases

ModBase

A1BDZ0.

Ontologies

GO:0005737;	Cellular component: cytoplasm (inferred from electronic annotation from HAMAP).
GO:0046857;	Molecular function: oxidoreductase activity, acting on other nitrogenous compounds as donors, with NAD or NADP as acceptor (inferred from electronic annotation from HAMAP).
GO:0033739;	Molecular function: queuine synthase activity (inferred from electronic annotation from EC).
GO:0008616;	Biological process: queuosine biosynthetic process (inferred from electronic annotation from HAMAP).
QuickGo view.

Family and domain databases

HAMAP

MF_00818; -; 1.
PBIL [Tree]

InterPro

IPR016856; CN_OxRdtase_NADPH-dep_QueF.
IPR001474; GTP_CycOHase_I.
Graphical view of domain structure.

Pfam

PF01227; GTP_cyclohydroI; 1.
Pfam graphical view of domain structure.

PIRSF

PIRSF027377; Nitrile_oxidored_QueF; 1.

ProDom

PD003330; GTP_cyclohydroI; 1.
[Domain structure / List of seq. sharing at least 1 domain]

TIGRFAMs

TIGR03139; QueF-II; 1.

BLOCKS

A1BDZ0.

Genome annotation databases

GeneID

4569428; -.

GenomeReviews

CP000492_GR; Cpha266_0561.

KEGG

cph:Cpha266_0561; -.

NMPDR

fig|290317.7.peg.589; -.

CMR

A1BDZ0; Cpha266_0561.

Other

ProtoNet

A1BDZ0.

UniRef

View cluster of proteins with at least 50% / 90% / 100% identity.

Keywords

Complete proteome; Cytoplasm; NADP; Oxidoreductase; Queuosine biosynthesis.

Features

Feature table viewer

`Key`	`From To`	`Length`		`Description`	`FTId`
`CHAIN`	`1 116`	`116`		`NADPH-dependent 7-cyano-7-deazaguanine reductase.`	`PRO_1000062382`

Sequence information

Length: 116 AA [This is the length of the unprocessed precursor]

Molecular weight: 13416 Da [This is the MW of the unprocessed precursor]

CRC64: BBA7CF4E27C8676A [This is a checksum on the sequence]

        10         20         30         40         50         60 
MKKEILEVFD NTFPGRDYTI EIVNPEFTSV CPITALPDFG TIIIRYIPDK SCVELKSLKY 

        70         80         90        100        110 
YFLEFRNAGI FYENITNTIL DDLTSVLQPR EMTVITQWKA RGGITETVSV TWPQKQ

A1BDZ0 in FASTA format

View entry in original UniProtKB/Swiss-Prot format
View entry in raw text format (no links)
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	BLAST submission on ExPASy/SIB or at NCBI (USA)		Sequence analysis tools: ProtParam, ProtScale, Compute pI/Mw, PeptideMass, PeptideCutter, Dotlet (Java)
	ScanProsite, MotifScan		Submit a homology modeling request to SWISS-MODEL
	NPSA Sequence analysis tools