NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
DOI=10.1128/JB.01726-06; PubMed=17293413 [NCBI, ExPASy, EBI, Israel, Japan]
Johnson T.J., Kariyawasam S., Wannemuehler Y., Mangiamele P., Johnson S.J., Doetkott C., Skyberg J.A., Lynne A.M., Johnson J.R., Nolan L.K.;
"The genome sequence of avian pathogenic Escherichia coli strain O1:K1:H7 shares strong similarities with human extraintestinal pathogenic E. coli genomes.";
J. Bacteriol. 189:3228-3236(2007).

Comments

FUNCTION: Catalyzes the NADPH-dependent reduction of 7-cyano-7-deazaguanine (preQ0) to 7-aminomethyl-7-deazaguanine (preQ1) (By similarity).
CATALYTIC ACTIVITY: 7-aminomethyl-7-carbaguanine + 2 NADP⁺ = 7-cyano-7-carbaguanine + 2 NADPH.
PATHWAY: tRNA modification; queuosine-tRNA biosynthesis .
SUBCELLULAR LOCATION: Cytoplasm (Probable).
SIMILARITY: Belongs to the GTP cyclohydrolase I family. QueF type 2 subfamily.

Copyright

Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.

Cross-references

Sequence databases

EMBL

CP000468; ABJ02221.1; -; Genomic_DNA.

[EMBL / GenBank / DDBJ] [CoDingSequence]

RefSeq

YP_854010.1; -.

3D structure databases

ModBase

A1AEY1.

Ontologies

GO:0005737;	Cellular component: cytoplasm (inferred from electronic annotation from HAMAP).
GO:0046857;	Molecular function: oxidoreductase activity, acting on other nitrogenous compounds as donors, with NAD or NADP as acceptor (inferred from electronic annotation from HAMAP).
GO:0008616;	Biological process: queuosine biosynthetic process (inferred from electronic annotation from HAMAP).
QuickGo view.

Family and domain databases

HAMAP

MF_00817; -; 1.
PBIL [Tree]

InterPro

IPR016428; CN_OxRdtase_NADPH-dep_YqcD.
IPR001474; GTP_CycOHase_I.
Graphical view of domain structure.

Pfam

PF01227; GTP_cyclohydroI; 1.
Pfam graphical view of domain structure.

PIRSF

PIRSF004750; Nitrile_oxidored_YqcD_prd; 1.

TIGRFAMs

TIGR03138; QueF; 1.

BLOCKS

A1AEY1.

Genome annotation databases

GeneID

4494418; -.

GenomeReviews

CP000468_GR; Ecok1_27270.

KEGG

ecv:APECO1_3737; -.

CMR

A1AEY1; Ecok1_27270.

Other

ProtoNet

A1AEY1.

UniRef

View cluster of proteins with at least 50% / 90% / 100% identity.

Keywords

Complete proteome; Cytoplasm; NADP; Oxidoreductase; Queuosine biosynthesis.

Features

Feature table viewer

`Key`	`From To`	`Length`		`Description`	`FTId`
`CHAIN`	`1 282`	`282`		`NADPH-dependent 7-cyano-7-deazaguanine reductase.`	`PRO_1000062340`

Sequence information

Length: 282 AA [This is the length of the unprocessed precursor]

Molecular weight: 32557 Da [This is the MW of the unprocessed precursor]

CRC64: 575A0751641EB5C3 [This is a checksum on the sequence]

        10         20         30         40         50         60 
MSSYANHQAL AGLTLGKSTD YRDTYDASLL QGVPRSLNRD PLGLKADNLP FHGTDIWTLY 

        70         80         90        100        110        120 
ELSWLNAKGL PQVAVGHVEL DYTSVNLIES KSFKLYLNSF NQTRFNNWDE VRQTLERDLS 

       130        140        150        160        170        180 
TCAQGKVSVA LYRLDELEGQ PIGHFNGTCI DDQDITIDNY EFTTDYLENA TSGEKVVEET 

       190        200        210        220        230        240 
LVSHLLKSNC LITHQPDWGS IQIQYRGRQI DREKLLRYLV SFRHHNEFHE QCVERIFNDL 

       250        260        270        280 
LRFCQPEKLS VYARYTRRGG LDINPWRSNS DFVPSTTRLV RQ

A1AEY1 in FASTA format

View entry in original UniProtKB/Swiss-Prot format
View entry in raw text format (no links)
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	BLAST submission on ExPASy/SIB or at NCBI (USA)		Sequence analysis tools: ProtParam, ProtScale, Compute pI/Mw, PeptideMass, PeptideCutter, Dotlet (Java)
	ScanProsite, MotifScan		Submit a homology modeling request to SWISS-MODEL
	NPSA Sequence analysis tools