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| Kingdom | Residues | Position | Rule |
| all | C,K,R,H,D,E,N,Q | anywhere (but not N-terminus) | - |
| Eukaryotes | P,A | N-terminus | - |
| Prokaryotes | A | N-terminus | - |
| Prokaryotes | F,Y,I,L,M | N-terminus after cleavage of signal peptide | rule PS00409: Consensus pattern [KRHEQSTAG]-G-[FYLIVM]-[ST]-[LT]-[LIVP]-E-[LIVMFWSTAG](14) |
| METH | DIMETH | TRIMETH | |
|---|---|---|---|
| average | 14.0269 | 28.0538 | 43.09 |
| monoisotopic | 14.0157 | 28.0314 | 43.0548 |
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